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Old 11-22-2015, 02:43 AM   #1
superpyrin
Junior Member
 
Location: US

Join Date: Aug 2014
Posts: 7
Default insert size bias

We have 3 different lengths of inserts: ~700, ~500 and ~275 bp and we consider mixing them in one lane in HiSeq. Is it known, or is it possible to estimate what is the bias in favor of shorter inserts? Is the 700 bp insert going to be completely overwhelmed by shorter ones?
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