View Single Post
Old 11-25-2015, 07:13 AM   #6
MU Core
Member
 
Location: Columbia, Missouri

Join Date: Apr 2008
Posts: 57
Default

superpyrin, the bias toward the small insert size has been our own personal experience as well. I've observed a 20% skewing of the read count toward the smaller fragment size (350 vs. 550) when both library were loaded at equal ratios. You'll want to perform a pooling approach as pmiguel suggest if pooling all three libraries together.
MU Core is offline   Reply With Quote