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Old 05-27-2016, 06:53 AM   #11
Bioo Scientific
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Location: Austin, Tx

Join Date: Oct 2009
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Hi Dan,

Small RNA sequencing is a little different in that it involves the ligation of single stranded adapters directly to RNA molecules, rather than conversion to dsDNA and ligation of Y-shaped adapters, as is used in other RNA-Seq kits, such as the TruSeq kits and the NEXTflex Rapid Directional RNA-Seq Kits. Because of this, the sequence reported is that of the RNA molecule, or the + strand. Consequently direct ligation also allows retention of the 5' and 3' most bases of the RNA molecule, which typically isn't possible with dsDNA ligation strategy, as some of that information is lost during first and second strand synthesis.
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