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Old 06-29-2016, 11:56 AM   #1
Location: New York

Join Date: Dec 2012
Posts: 14
Unhappy Quantitation Variations with Qubit

Hello All,

I have run into quite a strange issue recently.

I have successfully and consistently been running 16S and 18S libraries and quantifying the final multiplexed pool using the Qubit. Recently I used a different assay then normal on the Qubit (BR instead of HS) and had a failed sequencing run. After going back and re-quantifying I saw that the BR and HS were yielding two different concentrations and always a twofold difference. BR always 2x higher than the HS reading. From our sequencing results, it also seems as the HS is accurate for the 18S sample while the BR is accurate for the 16S.

Has anyone encountered this before or have any idea as to what could cause this?

Thank you!
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