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Old 07-01-2016, 05:12 AM   #5
MU Core
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Location: Columbia, Missouri

Join Date: Apr 2008
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Consistency can be an issue. Our group performs two independent HS Qubit readings per library. Doing so will help catch any outlier measurements due to a pipetting error, etc. The average is then used between the two readings for nM calculation. Using the average fragment size has been shown in our hands to be more consistent as well. Although this is less of an issue for amplicons.
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