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Old 03-29-2017, 08:28 AM   #3
ATϟGC
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Location: Canada

Join Date: Jun 2013
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The best method might depend largely on the taxon or taxa you are using if there are abundant co-precipitating contaminants. I have attached a method I developed/modified for HMW extraction of DNA from polysaccharide-rich tissues for PacBio sequencing (DOI:10.1111/1755-0998.12616)

I have not tried either of the two protocols described below but they might also be of interest to you:

http://enseqlopedia.com/2017/03/hmw-...e-10xgenomics/
Attached Files
File Type: pdf Steeves et al_MER-MoLSC_HMW_DNA_extraction_method.pdf (134.1 KB, 57 views)
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