Thread: MiSeq v3 600
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Old 10-31-2017, 02:35 AM   #1
ATGCT
Junior Member
 
Location: Austria

Join Date: Oct 2017
Posts: 4
Default MiSeq v3 600

Hi everyone,

we have troubles with our MiSeq Runs. At about cycle 80 we have a massive decrease in Q30scores.In general the quality of the runs is not good. Q30 scores of 50 or lower. In addition, during my last two runs- the intensity does not increase . I am very new to this technique and have therefore attached some Plots from basespace.
Maybe someone had the same problem? Or has an idea about what is going wrong- what are the possible causes? I went through the troubleshooting guides, but could find yet any answer.

thanks :-)
Attached Images
File Type: png Intensities.png (75.1 KB, 39 views)
File Type: png Run Overview.png (103.0 KB, 45 views)
File Type: png Run Overview II.png (56.5 KB, 37 views)
File Type: png Q30 score drop.png (43.3 KB, 40 views)
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