Thread: MiSeq v3 600
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Old 10-31-2017, 04:14 AM   #3
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Location: Bethesda MD

Join Date: Oct 2009
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The run is significantly over-clustered, as indicated by the low PF (only 68%; should be mid-80s to mid-90s) in combination with the high error rate (4.4%; should be <1%). Quantify your libraries by qPCR and load at the recommended concentration (actually, a bit lower for PE-300bp runs).
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