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Old 12-01-2017, 05:06 AM   #7
DPVT
Junior Member
 
Location: Acela Corridor

Join Date: Dec 2017
Posts: 3
Default qPCR/ Qubit discrepency

Our lab has often encountered trouble quantifying our Illumina pools. We sequence all manner of samples and find that some pools cluster more robustly then other others even when loaded at the same molarity. As a work around we load pools at different concentrations according how different the qPCR concentration and qubit HS concentration is.
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