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Old 05-04-2018, 04:47 AM   #9
MU Core
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Location: Columbia, Missouri

Join Date: Apr 2008
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chanwu,

We trust both measurements but use each for different library types. qPCR is used for PCR-free library preps with all other libraries which undergo amplification being measured by Qubit. The key is to find the method that works best for you and will allow you to normalize your loading of libraries. For example, we measure all RNA-Seq library preps by Qubit and know that our target loading density is consistent on the NextSeq with 0.9 pM. If you vary the method, however, you'll definitely see loading variability.
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