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Old 05-21-2018, 07:59 AM   #14
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Location: Acela Corridor

Join Date: Dec 2017
Posts: 3

So for our qPCR we do 20,000x dilutions of our pools to achieve a dilution in the middle of the stnd curve (around 0.2pM) and do two dilutions of 1000x and 5000x of the internal standard 0. however much the average of those two standards is off from 200pM is how much we normalize the calculated concentration of our pools by
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