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Old 02-20-2019, 06:37 PM   #5
SDPA_Pet
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Join Date: Apr 2013
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Quote:
Originally Posted by nucacidhunter View Post
Paired end sequencing means that library fragments (in this case ITS amplicons) are sequenced from both ends so R1 and R2 of the fragment are in opposite direction. If sequencing cycles are longer than library fragment then R1 nad R2 can be merged (if there is enough overlap). You can ask the sequencing centre to provide at least the workflow and that should answer your question.
ôR1 and R2 of the fragment are in oppossite direction". -- I know this. However, my question is when they sequence R1 and R2, do they have to use two primers? For example, R1 uses forward primer and R2 uses reverse primer.

Or they even don't have to use the PCR primer? They use universal primers?
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