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Old 05-07-2019, 12:01 PM   #1
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Location: Purdue University, West Lafayette, Indiana

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Default Best method to add custom primer to MiSeq Illumina sequencing primers?

What pipette is recommended (or have you seen used) to pipette custom primer into the Illumina sequencing primer wells of a MiSeq? (See here :

if it isn't clear what I mean.)

Currently we use some 1ml glass serological pipettes that were purchased over a decade ago. They actually work, but only poorly. We pipet out the liquid in Cartridge Positions 12 and/or 13 and/or 14 into eppendorf tubes. Mix in the (concentrated) custom primers to 0.5uM and pipet the mixture back into the position from which it came.
But if there existed a long pipette tip that fit on the end of a normal pipetter (p1000), that would be preferable.

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