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Old 08-12-2011, 02:07 AM   #1
gavin.oliver
Senior Member
 
Location: uk

Join Date: Jan 2010
Posts: 110
Default Sequencing and genotyping one gene

Can anyone recommend how best to sequence a single gene (25KB) for SNP/Indel-calling and genotyping purposes.

Sanger is fine for sequencing the gene once but multiple copies are required for the application and it quickly becomes very expensive.

Most sequencing centres seem to tend towards their HiSeqs which would mean 1500 x coverage or something similarly crazy (and that is with multiplexing).

Can anyone suggest an alternative and/or a provider?

Thanks in advance.

Last edited by gavin.oliver; 08-12-2011 at 02:12 AM.
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