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Old 05-24-2017, 09:37 AM   #5
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 234

In your sample sheet have you reverse complimented your i7 sequences? i5 is sequenced 5'->3', i7 is sequenced 3'->5'
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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