Hi,
I am currently doing this mouse cells and human cells co-culture and I am looking at the mutual influence of these 2 types of cells on each other. I want to do RNA-Seq on both the mouse and human cells. However, the co-culture is somehow hard to separate to purity and any purification attempt would lead to changes in gene expression. I am wondering if I could seq the mouse and human samples together in one single lane or using a single barcode. In that way, my sample will be a mixed of human and mouse DNA. I wonder how hard is it to do the actual alignment (correctly to the correct species) and is my experiment plan is feasible at all.
Thanks!
I am currently doing this mouse cells and human cells co-culture and I am looking at the mutual influence of these 2 types of cells on each other. I want to do RNA-Seq on both the mouse and human cells. However, the co-culture is somehow hard to separate to purity and any purification attempt would lead to changes in gene expression. I am wondering if I could seq the mouse and human samples together in one single lane or using a single barcode. In that way, my sample will be a mixed of human and mouse DNA. I wonder how hard is it to do the actual alignment (correctly to the correct species) and is my experiment plan is feasible at all.
Thanks!
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