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Old 04-18-2017, 07:47 AM   #1
jalinaodb
Junior Member
 
Location: Antwerp

Join Date: Apr 2017
Posts: 1
Question Priming MinION flow cell

Hi!

I've been working with the MinION from Oxford Nanopore for a small project. I used the Rapid Sequencing Kit (RAD002) and followed the protocol from the Rapid Lambda Control Experiment. I've been going through the protocol step by step, trying to understand all the steps. Most of them I've figured out. One step however I don't fully understand: Priming the flow cell.

Before loading your library you need to prepare a priming mix, containing RBF and nuclease-free water. First you load an amount into the priming port, then you load some into the flow cell via the SpotOn port. Why is this necessary? What exactly does this priming mix do?

Another question: are the Library Loading Beads really necessary? Or can you get the same results without them?

Hope someone can help!
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