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Old 04-18-2017, 11:25 AM   #2
gringer
David Eccles (gringer)
 
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 838
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Quote:
What exactly does this priming mix do?
I can't give you an exact answer, but my understanding is that the priming mix includes ATP and additional chemicals that improve the rate of sequencing. The second flush of the priming mix (with the SpotON port open) helps to clear any blockages near the SpotON port and generate a bit of suction to help with the drip loading -- the closer this is done to sample loading the better.

Quote:
are the Library Loading Beads really necessary? Or can you get the same results without them?
Loading beads bring the DNA closer to the pores prior to the start of sequencing. The loading beads aren't necessary for sequencing to be carried out, but they are necessary for high-yield (and high-speed) sequencing. The same results will not be achieved without library loading beads.
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