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Old 06-03-2020, 08:44 AM   #1
SofiaGranja
Junior Member
 
Location: Oxford

Join Date: May 2020
Posts: 4
Lightbulb TET,EBT, Tween-20

Hi everyone,

I have two questions:

There are a considerable number of DNA extraction protocols that indicate to elute in TET (10 mM Tris – HCl + 1 mM EDTA + 0.05% Tween 20). However, the presence of EDTA can inhibit future reactions such as library build. Any idea why the protocols are not standardised to always use EBT in the elution?

Regarding to Tween 20, it is a non-ionic detergent that reduces adsorption of DNA to plastics and improves pipetting accuracy. However, if we use low binding tubes, adding tween 20 to TE or EB, it is not redundant or even useless to use it? (since the technology that these optimized tubes present nearly 100% recovery of DNA / RNA molecules).

Can't wait to hear your thoughts on this.

Thank you,
Sofia

Last edited by SofiaGranja; 06-03-2020 at 08:46 AM.
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