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Old 12-04-2015, 07:24 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,231
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I do not have direct experience with your query. Beads have a binding capacity that can be found in manufacturer specification sheet and quantity or number of transcripts that are depleted can be estimated. Using twice the estimated amount is a good starting point.

On the subject of rRNA depletion there are other products which are more cost effective:

1- Lexogen RiboCop (works by hybridisation of RNA to probes and binding probes to beads similar to Ribo-Zero Gold)
2- RNaseH based degradation product from NEB
3- Clontech SMARTer products with kit included RiboGone (RNaseH based) reagent. These products have a considerable time-saving advantage as well because they have ligation-free workflow
4- Post-cDNA synthesis depletion workflows

Last edited by nucacidhunter; 12-11-2015 at 01:28 AM.
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