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Old 07-03-2018, 10:53 PM   #1
Drmadscientist
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Location: India

Join Date: May 2014
Posts: 1
Default Question on multiplexing - long reads

Hi All

So I have 2-3 number of 2MB bacterial genome and two plasmids of 17 kb each length. I am looking at the feasilbility of multiplexing them in a single SMRT or MinIon flow cell. Has anyone ever multiplexed plasmids with genomic DNA?

Also, if you look at the data requirement, I would like say 1000x for both but then for PacBio they suggest you start with equal amounts of DNA for shearing. That will probably mean that I will get 1000x of bacterial genome and maybe 10000x of plasmid sequence which I do not want .

Same goes for MinIon multiplexing.

In Illumina you could do your adjustments for data requirement when you dilute your sequencing pools. Is the same possible here and has anyone tried?

Thanks
DMS
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