Do you think there are any applications for which Sanger sequencing (i.e. capillary electrophoresis, CE) still holds a competitive advantage over Next-Gen technologies?
I thought that as of a few years ago,
1. Sanger was still needed for sequencing gaps between the short reads of NGS, and
2. NGS was more error-prone than Sanger, and in particular NGS didn't do well with PCR errors e.g. for sequences containing short tandem repeats
But I don't know if that's still true, and I hear that MiSeq has nailed the lid on the coffin for Sanger.
Are there applications for which researchers still need Sanger sequencing as a follow-up to their NGS results?
For users who still maintain their CE machines (e.g. ABI 3730, etc), what do you use them for? I also saw some reports from a few years ago that CE machines could be re-purposed for glycosylation profiling instead of DNA sequencing. Any other creative ideas for soliciting samples to keep the machine running?
Thanks for sharing your ideas!
I thought that as of a few years ago,
1. Sanger was still needed for sequencing gaps between the short reads of NGS, and
2. NGS was more error-prone than Sanger, and in particular NGS didn't do well with PCR errors e.g. for sequences containing short tandem repeats
But I don't know if that's still true, and I hear that MiSeq has nailed the lid on the coffin for Sanger.
Are there applications for which researchers still need Sanger sequencing as a follow-up to their NGS results?
For users who still maintain their CE machines (e.g. ABI 3730, etc), what do you use them for? I also saw some reports from a few years ago that CE machines could be re-purposed for glycosylation profiling instead of DNA sequencing. Any other creative ideas for soliciting samples to keep the machine running?
Thanks for sharing your ideas!
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