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Old 08-16-2017, 05:35 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,179
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50% loss is too much; an acceptable loss is 10-15% and 3x bead is also excessive. You are using too much DNA to start with so if you go to PCR (multiple 20 ul reactions) with 2 ng of adapted size selected tags and 5% is amplifiable then up to 12 cycle should produce more than enough library for QC and sequencing.
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