Hello,
This is my first time to construct an Illumina Paired-end library. According to the protocols, there is a PCR purification step after ligation of adapters and prior to size selection step. If I want to perform the gel size-selection before PCR enrichment, is this PCR purification step necessary?
I guess I can directly run the ligation product on a gel for size selection, but both Illumina native kit and NEBNext kit include that PCR purification step?
Best wishes,
SK
This is my first time to construct an Illumina Paired-end library. According to the protocols, there is a PCR purification step after ligation of adapters and prior to size selection step. If I want to perform the gel size-selection before PCR enrichment, is this PCR purification step necessary?
I guess I can directly run the ligation product on a gel for size selection, but both Illumina native kit and NEBNext kit include that PCR purification step?
Best wishes,
SK
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