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seq_me · 07-06-2011, 11:45 PM

Yes t4 polymerase will make the ends blunt and T4 PNK will make sure that 5' ends are phosphorylated and 3' ends have the hydroxyl group. I dont think of a reson of not using this esp if your DNA fragments are generated using sonication

07-06-2011, 11:45 PM	#4
seq_me Junior Member Location: San Francisco Join Date: Feb 2010 Posts: 8	Yes t4 polymerase will make the ends blunt and T4 PNK will make sure that 5' ends are phosphorylated and 3' ends have the hydroxyl group. I dont think of a reson of not using this esp if your DNA fragments are generated using sonication