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Old 07-06-2011, 11:45 PM   #4
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Location: San Francisco

Join Date: Feb 2010
Posts: 8

Yes t4 polymerase will make the ends blunt and T4 PNK will make sure that 5' ends are phosphorylated and 3' ends have the hydroxyl group. I dont think of a reson of not using this esp if your DNA fragments are generated using sonication
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