View Single Post
Old 05-24-2019, 01:23 PM   #7
thermophile
Senior Member
 
Location: CT

Join Date: Apr 2015
Posts: 241
Default

is your cluster density the same top and bottom of the flow cell? if you have sig more reads on the bottom you may be carrying over etoh from the ampure cleanup
__________________
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
thermophile is offline   Reply With Quote