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Old 01-27-2010, 07:39 AM   #27
mccullou
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Location: CA

Join Date: Oct 2008
Posts: 13
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So are most buffers if you ask qiagen or invitrogen, but we are all smart people here, right? It is either photo or chemical cleavage and looking at all the steps in the sequencing process there doesn't seem to be a hint. I am just curious because I have been having increasing background and phase issues and I think it might be inefficient removal of that flourophore/blocking agent, but I can't figure it out my issue if I don't know how the base is removed. . .
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