View Single Post
Old 05-24-2010, 03:05 AM   #59
Location: uk

Join Date: Apr 2010
Posts: 43

I ordered the following primers from Sigma:



*=Phosphorothioate bond

However I can't get them working with my ChIP input DNA sample. Have tried varying the number of cycles, but each time after the PCR amplification of my library I only have 2 or 3 ng/ul, whereas colleagues have had more like 20 ng/ul.

I'm unsure whether the problem is with the primers or something else. As far as I know, everything apart from the primers is the same as colleagues' libraries that worked. I don't have any Illumina primer to test alongside.
AdamB is offline   Reply With Quote