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Old 01-06-2017, 07:27 AM   #6
Location: NE, USA

Join Date: Jun 2016
Posts: 14

Hi Yepler, thanks for your response with suggestions. As you mentioned, my Tm is pretty low when you checked it on IDT oligo analyzer. I looked it on exiqon, it is close to 70C. I can do LNA to increase Tm of my sequencing primer up to ~74C based on the exiqon calculation, but will still be 65C on IDT. So assume, I just do LNA and trust Tm with exiqon. Any other suggestions? such as length of oligo and position of LNA. Thank you again.

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