Hi everyone,
I need a help please. I need to verify my understanding of the difference between cDNA and CDS.
From the definition, CDS is a region of nucleotide that corresponds to the sequence of aa in the predicted protein . The CDS contains start & stop codon and does not include any UTR and introns. Therefore, CDS does not correspond to the actual mRNA sequence.
On the other hand, cDNA is the DNA version of mature mRNA (ie, does not include introns, but include the UTR, such as Kozak sequence etc).
Am I right?
One of the web that I was reading (I forgot which one, but it's a reliable source) mentions that CDS should start with a start codon. However, when I was browsing through NCBI, one of the nucleotide sequences of a protein that I found says "complete cds" does not start with ATG. And this nucleotide sequence does not correspond to the translated protein seq (from the same author). Why is that?
Any help is appreciated. Thank you so much.
regards,
s13ep
I need a help please. I need to verify my understanding of the difference between cDNA and CDS.
From the definition, CDS is a region of nucleotide that corresponds to the sequence of aa in the predicted protein . The CDS contains start & stop codon and does not include any UTR and introns. Therefore, CDS does not correspond to the actual mRNA sequence.
On the other hand, cDNA is the DNA version of mature mRNA (ie, does not include introns, but include the UTR, such as Kozak sequence etc).
Am I right?
One of the web that I was reading (I forgot which one, but it's a reliable source) mentions that CDS should start with a start codon. However, when I was browsing through NCBI, one of the nucleotide sequences of a protein that I found says "complete cds" does not start with ATG. And this nucleotide sequence does not correspond to the translated protein seq (from the same author). Why is that?
Any help is appreciated. Thank you so much.
regards,
s13ep
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