SEQanswers - View Single Post - (2x) Final Bead Clean-Up

luc · 10-30-2019, 04:34 PM

Yep, bead cleanups will never be perfect. You can only enrich for molecules of the desired size range.
Many protocols suggest two rounds of bead cleanups likey to safeguard for less than optimal experiments that created too many adapter dimers.

10-30-2019, 04:34 PM	#3
luc Senior Member Location: US Join Date: Dec 2010 Posts: 453	Yep, bead cleanups will never be perfect. You can only enrich for molecules of the desired size range. Many protocols suggest two rounds of bead cleanups likey to safeguard for less than optimal experiments that created too many adapter dimers.