Thread: A-base addition
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Old 01-16-2010, 09:07 PM   #3
shurjo
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Location: Rockville, MD

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If recessed 3' ends are present, Klenow exo- fills them in and leaves a single base overhang in ~50% of cases. Given the blunt ends from the previous step in the Illumina protocol, it simply adds a single A to the 3' end.
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