Thread: A-base addition
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Old 11-01-2009, 03:40 PM   #1
seqgirl123
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Join Date: Oct 2008
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Default A-base addition

In the polishing step for library preparation, there is a step using Klenow exo- polymerase and dATP, which will add one A-base to the 3' end of blunt ended DNA.

My question is how does only one A-base end up getting added onto the 3' end and not two or three?
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