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Old 03-15-2017, 05:08 AM   #1
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Location: Oxford, Ohio

Join Date: Mar 2012
Posts: 246
Default Amplicon Sequencing & Reads Per Sample (Coverage)


Is there a "best" way to normalise and pool amplicon samples to acquired even, or balanced read coverage. Otherwise known as getting the same "reads per sample" for each of the pooled samples? We often end up with stochastic coverage despite our best efforts. I'm looking for a better way...

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