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Old 03-15-2017, 01:44 PM   #5
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 243

my facility has a capillary fragment analyzer (qiaxcel, ~$0.40/sample not considering machine cost). I pool eqimolar based on that, then clean the entire pool in a single spri cleanup. I'm generally happy if I don't have many samples with an order of magnitude more sequences than I'm aiming for. I didn't buy the qiaxcel and if I didn't have it, I'd probably do what kmcarr does. I totally agree that I'm concerned with getting enough reads per sample and don't worry about the few winners of the pooling lottery. I run on MiSeq so the few samples that don't get enough seqs can just be repooled for the next run.
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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