Tweet
I am trying to determine the method used to generate my RNAseq data. On the Illumina website, the read length are either 1*36bp or 2*50bp. My fastq reads are non-strand-specific and non-paired sequences of 50bp each. I'm not sure how to interpret this...
-
-
The HiSeq platform can generate reads of various lengths, strand-specific or non-strand-specific, paired or unpaired; 1x50 and 2x50 runs are both possible. It might help if you posted the first two reads (first 8 lines) of the fastq file here, but I'm not really sure what your question is. -
Illumina has various kits for RNAseq library prep. As Brian has pointed, libraries from all kits can be sequenced for various length and also as single read or paired read. Core centre or person preparing libraries should be able to provide information on kit and method used for library prep.Comment
-
That make sense. Thanks!Comment
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment