Hello all,
I will be generating 16S amplicons for use on the MiSeq platform. The article that I am basing my protocol on notes that they used the MoBio PCR clean-up kit to remove residual PCR "junk" from their amplicon products prior to sequencing. The kit is rather expensive for my lab and I know that we already have the Qiagen PCR Cleanup kit. Does anyone have any recommendations on which kit to use? How different are the kits from one another? Does one work better than the other? Will I get similar results?
Thanks,
Rachael
I will be generating 16S amplicons for use on the MiSeq platform. The article that I am basing my protocol on notes that they used the MoBio PCR clean-up kit to remove residual PCR "junk" from their amplicon products prior to sequencing. The kit is rather expensive for my lab and I know that we already have the Qiagen PCR Cleanup kit. Does anyone have any recommendations on which kit to use? How different are the kits from one another? Does one work better than the other? Will I get similar results?
Thanks,
Rachael
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