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Old 03-20-2017, 05:03 PM   #5
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,189
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This is the limitation of using fusion primers. Using gel purified primers for both PCR steps and sequencing will improve performance by minimizing miss-synthesized oligos containing indels and mismatches.
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