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Old 03-12-2015, 04:35 PM   #3
luc
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Location: US

Join Date: Dec 2010
Posts: 430
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Sorry, we never figured out what went wrong. Clearly there was a problem with the library prep (two sequencing runs yielded the same type of unusable barcode data). The postdoc re-made the libraries (using the same protocol - standard genomic DNA library prep using NEB and Enzymatics enzymes; Bioo adapters) and the results where problem-free.
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