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Old 03-12-2015, 04:50 PM   #4
rogerzzw
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Location: Grand Rapids

Join Date: Mar 2015
Posts: 16
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Thanks, luc.

What we did is pooling several different libraries (made by two persons) together to get sequence. It turned out about 30% of reads did not match any of the barcodes used in library prep. 6bp G accounts for a fair amount of reads that did not get recognized.

It looks more difficult here because you probably do not know who made it run. Maybe we can try to align the reference genome ( one person use human sample, the other use mouse sample), to see where it belongs to. So that maybe we can know.

Thanks!
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