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Old 02-24-2014, 02:14 AM   #2
Location: London

Join Date: Nov 2012
Posts: 96

I know that some people on this forum just add some padding nucleotides before the adapter, so you can extend the sequencing primer back into that, bringing your Tm up. I was certainly once told by an Illumina rep that they'd be wary of engineering overlap between the lawn oligos and sequencing primers.
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