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Old 11-13-2014, 02:37 PM   #2
DNA_Dan
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Location: Montana

Join Date: Nov 2008
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I think Illumina recommends a two-step approach. Fusion primers with barcode and sequencing primer locations, then a second PCR with P5/P7 ends being added. If you do it all at once it may get ugly. Also if you are low diversity adding in a N+1, N+2 linker in there greatly helps diversity.
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