SEQanswers - View Single Post - 'Assembly PCR' for homemade amplicon library prep

ScottC · 11-13-2014, 02:48 PM

Try this:

http://supportres.illumina.com/docum...15044223-b.pdf

Also, I'm not sure you need the extra N bases to increase diversity, if you're using the newest MiSeq software.

Cheers,

Scott.

11-13-2014, 02:48 PM	#3
ScottC Senior Member Location: Monash University, Melbourne, Australia. Join Date: Jan 2008 Posts: 246	Try this: http://supportres.illumina.com/docum...15044223-b.pdf Also, I'm not sure you need the extra N bases to increase diversity, if you're using the newest MiSeq software. Cheers, Scott.