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Old 11-13-2014, 08:44 PM   #7
DNA_Dan
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Location: Montana

Join Date: Nov 2008
Posts: 21
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As a rule of thumb, we never use the same barcode combination twice on a plate. It's not like you have the option of running the same barcodes in multiple lanes like you can on a HiSeq.

For us the most critical part is keeping your primer stocks contamination free prior to the PCR. After normalization and pooling you would have no way of knowing your DNA was tagged with multiple barcodes without some sort of internal control or verification of the target.

Pipette wisely!

Last edited by DNA_Dan; 11-13-2014 at 08:49 PM.
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