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Old 11-22-2014, 02:53 AM   #18
BioKiwi
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Location: KiwiTeritory

Join Date: Sep 2014
Posts: 19
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Thanks for clarifying your position on PCR contamination issues. I do not think that a mechanism or evidence has been provided for PCR contamination you have implied in earlier posts:
Quote:
Aerosols build in labs over time. In a post PCR area you can use ART tips, UV and gloves but this minimises contamination it won't remove it. A good PCR once 'opened' will generate aerosols with 10^5-10^9 copies of DNA that can travel through HEPA filters and build in a lab.
I was looking for evidence that shows an amplicon travels through HEPA filter, is not degraded by UV exposure, builds up in the lab and somehow ends up in another PCR reaction.
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