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Old 12-11-2014, 01:05 PM   #1
SeqVicious
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Location: New Hampshire

Join Date: Sep 2011
Posts: 20
Default Good RIN, bad mRNA?

Hello, I'm preparing some RNA for RNAseq and I submitted my samples to the local sequencing core for quality control on the bioanalyzer. The results came back and my samples have good RIN values, almost all >9. But I can't see a nice bump for the mRNA in which I am interested. I'm not sure if all of the peaks along the electrophoretogram are obscuring the mRNA. Should I trust these samples based on the RIN value alone? I want to proceed to ribosome depletion but I don't want to waste an expensive kit on samples that might not have good mRNA in them.

The attached is an example of my bioanalyzer results.

Thanks!
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File Type: pdf m9statbioan.pdf (612.2 KB, 71 views)
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