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Old 09-28-2017, 03:30 AM   #62
BioKiwi
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Location: KiwiTeritory

Join Date: Sep 2014
Posts: 19
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One library, primer annealing, polymerase binding, clean up and Magbead binding was done in one batch (one tube) then was splitted into three wells on the same sample plate and sequenced using the same SMRT Cell tray and sequencing reagent. P0, P1 and P2% and output is different for each Cell. It seems that variation is result of what happens inside the sequencer or SMRT cell quality.
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