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Old 07-23-2010, 06:20 AM   #1
JUdw
Junior Member
 
Location: The Netherlands

Join Date: Dec 2009
Posts: 9
Default In-solution SureSelect capture library to prepped sample ratio

I’m wondering about the in-solution SureSelect library to prepped sample ratio. The SureSelect protocol states “The ratio of SureSelect capture library to prepped library is critical for successful capture”. What ratio we’re talking about here? I suppose it is the molar bait to target ratio? So then when I have a 4x tilled bait design, can I decrease the Sureselect capture library weight by two? Furthermore, when I enrich a species like e.g. Arabidopsis, which is >10x smaller then human, then the molar bait to target ratio also increases doesn’t it?
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