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Old 04-17-2020, 06:35 AM   #3
Location: Illinois

Join Date: Oct 2014
Posts: 42

Could try purchasing some new Sodium Hydroxide and Tris for denaturing libraries. If your NaOH is over a year old and has been opened a lot, I'd recommend purchasing a new bottle. The lenses on the NextSeq get covered in dust over time. Usually a reduction in clustering due to lenses being dirty coincides with a lower PF rate.
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