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Old 08-18-2010, 08:35 AM   #2
Exeplex
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Location: Exeter, UK

Join Date: Jun 2009
Posts: 10
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Hi,

There is some useful information in the MYselect manual (a system not dissimilar to Agilent's SureSelect), where they actually give you such details as the bait concentration and numbers of molecules in the bait library versus target (details notably absent from any Agilent protocols).

Should be particularly useful if you are scaling your reactions up or down, doing multiplex capture reactions or using custom libraries.

The MYselect protocol manual can be downloaded from here:
http://www.mycroarray.com/products/myselect.html
and no, I don't work for them (although I'll probably be trying out their system for myself pretty soon)!

Good luck!
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